Hydrogen-induced damage is an inevitable challenge in pipeline safety applications, especially, the fusion welded joints owing to microstructure heterogeneity caused by welding process. In this work, X100 pipeline steel was subjected to friction stir welding (FSW) at rotation rates of 300-600 rpm under water cooling, and the relationship among the microstructure, hydrogen diffusivity, and hydrogen embrittlement (HE) behavior of the nugget zone (NZ) were studied. The NZ at 600 rpm had the highest effective hydrogen diffusion coefficient (Deff) of 2.1 x 10-10 m2/s because of the highest dislocation density and lowest ratio of effective grain boundary. The Deff decreased with decreasing rotation rate due to the decrease of dislocation density and the increase of ratio of effective grain boundary, and the lowest Deff of 1.32 x 10-10 m2/s was obtained at 300 rpm. After hydrogen charging, the tensile strength of all specimens decreased slightly, while the elongation decreased significantly. As the rotation rate decreased, the elongation loss was obviously inhibited, and ultimately a lowest elongation loss of 31.8% was obtained at 300 rpm. The abovementioned excellent mechanical properties were attributed to the fine ferrite/ martensite structure, low Deff, and strong {111}//ND texture dramatically inhibiting hydrogen-induced cracking initiation and propagation.(c) 2022 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.