The fabrication of multi-layer alloys by additive friction stir deposition (AFSD) results in a complicated microstructure and mechanical property evolution due to the repeated thermal inputs impacting the existing deposited layers. This work systematically studied the microstructure and mechanical properties of several areas (last layers, intermediate layers, and first layers) of a 16-layer 2195 alloy component fabricated by AFSD to ascertain the effect of repeated thermal cycling. The periodic heat input resulted in the minimal quantities of T 1-phase only appearing in the last layers of the sample, while the θ′-phase developed a complex precipitate with the δ′ and β′ phases. The mechanical properties of the 2195 sample exhibit a gradient development related to the microstructure, with a decrease in strength and hardness from top to bottom. The samples located in the last layers show the highest microhardness of 117.0 Hv, yield strength of 296.6 MPa, ultimate tensile strength of 440.6 MPa, and elongation of 27.1%, respectively.