One of the key challenges faced by Triboelectric Nanogenerators (TENGs) in the fields of wearable electronics and biomedical energy harvesting is achieving high output performance, durability, and cost-effectiveness. To address these challenges, a DNA-helix structured kirigami nanogenerator (DSKN) is developed, accommodating 28 hole-shaped power generation units within a compact space, resulting in exceptionally high output performance. Under impulse excitation from footsteps, it generates an instantaneous output of 1552 V and 31.04 μA, achieving a peak power density of 1.30 mW/cm 3, through ion beam etching and dip-etching technologies. Notably, it demonstrates a maximum elastic force of 34.5 N during a 24 mm compression-release displacement, requiring 132.4 mJ, indicating its potential for shock-absorbing applications. Building on these mechanical properties, we further integrated DSKN with alternating current electroluminescence (ACEL) to develop a flexible antibacterial insole that harvests mechanical energy from walking to power the ACEL device. The insole activates a photosensitizer (PS) to generate reactive oxygen species (ROS), effectively eliminating over 99 % of drug-resistant bacteria. This work not only enhances the output performance of TENGs but also demonstrates their potential in multifunctional applications, paving the way for next-generation self-powered and antibacterial technologies.